BIOPIC 学术报告
报告题目：Harnessing microfluidics in few-cell and single-cell manipulations for biological discovery 
报告人：Prof. Angela Wu
Division of Life Sciences 
Hong Kong University of Science & Technology
报告时间：2016年8月17日（星期三）上午10:00
报告地点：北京大学综合科研楼生物动态光学成像中心302会议室
摘要：
Interest in few-cell and single-cell analysis has been growing rapidly, especially as many biological questions can only be addressed by profiling rare or heterogeneous populations of cells (e.g., stem cells). However, using conventional techniques to analyze rare cells in microliter volumes poses challenges in experimental sensitivity and reproducibility. Microfluidic manipulation in small fluid volumes can both increase assay sensitivities and improve reproducibility through automation. In my work, I apply microfluidics to single cell analysis, I systematically evaluate the sensitivity, precision, and accuracy of various approaches to single-cell RNA-seq.  Our results show that it is possible to use single-cell RNA-seq to perform quantitative transcriptome measurements of single cells, and that microfluidic volumes help to reduce result bias. We subsequently used microfluidic-based RNA-seq to profile single cells of the developing lung in embryonic mice, as well as cells of primary human colon and cervix samples, and identified novel transcriptional markers for various subpopulations. These results obtained using microfluidics were some of the first in the field of single-cell RNA-seq, and heralded single-cell genomics as an indispensible tool in various biological and medical applications.
联系人：黄岩谊（电话：62744058，Email：yanyi@pku.edu.cn）
欢迎各位老师同学积极参加！