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李毓龙

邮  箱: yulongli@pku.edu.cn

职  称:研究员

办公室地址:北京市海淀区颐和园路5号,北京大学,吕志和楼,100871

实验室地址:北京市海淀区颐和园路5号,北京大学,吕志和楼,100871

实验室主页:http://www.yulonglilab.org

  • 个人简介
  • 科研领域
  • 代表性论文

教育经历:

2000-2006年,博士,神经生物学系,杜克大学
1996-2000年,学士,生命科学学院,北京大学

荣誉奖励:

科学探索奖生命科学奖,2019
国家杰出青年科学基金,2019
第十二届谈家桢生命科学创新奖,2019
张香桐神经科学青年科学家奖,2019
中国十大医学科技新闻,2018
中国生命科学十大进展,中国科协生命科学学会联合体,2018
中源协和生命医学奖创新突破奖,2018
勃林格殷格翰研究员奖,2018
绿叶生物医药杰出青年学者奖,2015

工作经历:

2006-2012年,博士后,分子和细胞生理学系,斯坦福大学
2012-今,研究员,生命科学学院,生命联合中心,麦戈文脑研究所,北京大学

学术任职:

2019-至今,Journal of Neurochemistry,编委
2018-至今,美洲华人生物科学学会,会员
2001-至今,美国神经科学学会,成员

执教课程:

遗传学讨论课
整合科学实验课
生命科学强化挑战班文献讨论课
cls神经生物学track
ptn神经生物学track
高级神经生物学
        人的大脑由数十亿的神经元组成,后者又通过数万亿的突触组成复杂的神经网络。不同种类的神经元经过或远或近的投射,通过突触与其他神经元进行信息交流,实现感知觉、决策和运动等高级神经功能。
研究大脑的最大挑战在于脑的高度复杂性。我们实验室集中在神经元通讯的基本结构突触上,从两个层面上开展研究:一是开发前沿的工具,即开发新型成像探针,用于在时间和空间尺度上解析神经系统的复杂功能;二是借助先进的工具探究突触传递的调节机制,特别是在生理及病理条件下对神经递质释放的调节。
具体而言,对于工具开发,我们集中于:
  1,结合光遗传学和荧光成像,无损伤性的研究神经元之间的电突触连接。电突触的异常可导致耳聋、癫痫、脑部肿瘤和心脏功能异常等疾病。
  2,开发可遗传编码的检测神经递质/调质的荧光探针。神经递质/调质是神经元化学突触传递的关键介导分子,与感知、学习和记忆以及情绪密切相关。
利用上述荧光探针,我们的功能性和生理性的研究集中于:
  1,结合双光子成像和可遗传编码的荧光探针,使用果蝇和小鼠作为模式生物,研究嗅觉传导或睡眠过程中脑的工作机制。
  2,寻找上述新型化学递质/调质小分子的对应受体,即寻找“孤儿”受体的配体。
  3,结合生物信息学、分析化学、生物化学、生理学和成像学方法,系统地探索和鉴定潜在的新型小分子神经递质。
Main Research Articles

· Yu, H., Zhao, T., Liu, S., Wu, Q., Johnson, O., Wu, Z., Zhuang, Z., Shi, Y., He, R., Yang, Y., Sun, J., Wang, X., Xu, H., Zeng, Z., Lei, X., Luo, W.* & Li, Y.*. (2019). MRGPRX4 is a bile acid receptor for human cholestatic itch. eLife, 8, e48431.
· Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J. & Li, Y.* (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine. Neuron, 102(4), 745-761.
· Wu, Z.#, Feng, J.#, Jing, M., & Li, Y.* (2019). G protein-assisted optimization of GPCR-activation based (GRAB) sensors. Neural Imaging and Sensing 2019, vol. 10865, p. 108650N. International Society for Optics and Photonics.
· Wu, L., Dong, A., Dong, L., Wang, S. Q., & Li, Y*. (2019). PARIS, an optogenetic method for functionally mapping gap junctions. eLife, 8, e43366.
· Sun, F.#, Zeng, J.#, Jing, M.#, Zhou, J., Feng, J., Owen, S., Luo, Y., Li, F., Wang, H., Yamaguchi, T., Yong, Z., Gao, Y., Peng, W., Wang, L., Zhang, S., Du, J., Lin, D., Xu, M., Kreitzer, A. C., Cui, G. & Li, Y.* (2018). A genetically-encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice. Cell, 174(2), 481-496.
· Jing, M.#, Zhang, P.#, Wang, G., Feng, J., Mesik, L., Zeng, J., Jiang, H., Wang, S., Looby, J. C., Guagliardo, N. A., Langma, L. W., Lu, J., Zuo, Y., Talmage, D. A., Role, L. W., Barrett, P. Q., Zhang, L. I., Luo, M., Song, Y., Zhu, JJ* & Li, Y*. (2018). A genetically-encoded fluorescent acetylcholine indicator for in vitro and in vivo studies. Nature Biotechnology, 36(8), 726-737.
· Li, Y.*, & Tsien, R. W.* (2012). pHTomato, a red, genetically encoded indicator that enables multiplex interrogation of synaptic activity. Nature neuroscience, 15(7), 1047-1053.
· Li, Y., Augustine, G. J., & Weninger, K.* (2007). Kinetics of complexin binding to the SNARE complex: correcting single molecule FRET measurements for hidden events. Biophysical journal, 93(6), 2178-2187.

Collaborative Publications

· Lin, R., Liang, J., Wang, R., Yan, T., Zhou, Y., Liu, Y., Feng, Q., Sun, F.,, Li, Y., Li, A., Gong, H., & Luo, M.* (2020). The Raphe Dopamine System Controls the Expression of Incentive Memory. Neuron, 1420-19.
· Zhang, X., Noyes, N. C. , Zeng, J., Li, Y. & Davis, R. L.* (2019). Aversive Training Induces Both Pre- and Postsynaptic Suppression in Drosophila. The Journal of Neuroscience, 1420-19.
· Handler, A., Graham, T. G. M., Cohn, R., Morantte, I., Siliciano, A. F., Zeng J., Li, Y. & Ruta, V.* (2019). Distinct dopamine receptor pathways underlie the temporal sensitivity of associative learning. Cell, 178(1), 60-75.
· Liang, X., Ho, M. C., Zhang, Y., Li, Y., Wu, M. N., Holy, T. E., & Taghert, P. H.* (2019). Morning and Evening Circadian Pacemakers Independently Drive Premotor Centers via a Specific Dopamine Relay. Neuron, 102(4), 843-857.
· Zhou, M.#, Chen, N.#, Tian, J., Zeng, J., Zhang, Y., Zhang, X., Guo, J., Sun, J., Li, Yulong., Guo, A.*, & Li, Yan.* (2019). Suppression of GABAergic neurons through D2-like receptor secures efficient conditioning in Drosophila aversive olfactory learning. PNAS, 201812342.
· Li, B.#, Wong, C.#, Gao, S. M., Zhang, R., Sun, R., Li, Y., & *Song, Y. (2018). The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking. eLife, 7, e38181.
· Tanaka, M., Sun, F., Li, Y., & Mooney, R.* (2018). A mesocortical dopamine circuit enables the cultural transmission of vocal behaviour. Nature, 563(7729), 117-120.
· Chen, B.#, Huang, X.#, Gou, D., Zeng, J., Chen , G., Pang, M., Hu, Y., Zhao, Z., Wu, H., Cheng, H., Zhang, Z., Xu, C., & Li, Y., Chen, L.*, Wang, A.* (2018). Rapid volumetric imaging with Bessel-Beam three-photon microscopy. Biomedical optics express, 9(4), 1992-2000.
· Shen, Y., Ge, W. P., Li, Y., Hirano, A., Lee, H. Y., Rohlmann, A., Missler, M., Tsien, R. W., Jan, L. Y., Fu, Y. H.* & Ptacek, L. J.* (2015). Protein mutated in paroxysmal dyskinesia interacts with the active zone protein RIM and suppresses synaptic vesicle exocytosis. Proceedings of the National Academy of Sciences, 112(10), 2935-2941.
· Liang, L., Li, Y., Potter, C. J., Yizhar, O., Deisseroth, K., Tsien, R. W., & Luo, L.* (2013). GABAergic Projection Neurons Route Selective Olfactory Inputs to Specific Higher-Order Neurons. Neuron, 79(5), 917-931.
· Park, H., Li, Y., & Tsien, R. W.* (2012). Influence of synaptic vesicle position on release probability and exocytotic fusion mode. Science, 335(6074), 1362-1366.
· Yoo, A. S.*, Sun, A. X., Li, L., Shcheglovitov, A., Portmann, T., Li, Y., Lee-Messer, C., Dolmetsch, R. E., Tsien R. W. & Crabtree, G. R.* (2011). MicroRNA-mediated conversion of human fibroblasts to neurons. Nature, 476(7359), 228-231.
· Zhang, Q., Li, Y., & Tsien, R. W.* (2009). The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles. Science, 323(5920), 1448-1453.
· Kuner, T.*, Li, Y., Gee, K. R., Bonewald, L. F., & Augustine, G. J. (2008). Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release. Proceedings of the National Academy of Sciences, 105(1), 347-352.


Reviews, Book Reviews and Highlights

· Wan, J. & Li, Y.* (2020). Recent Advances in Detection Methods for Neurotransmitters. Chinese Journal of Analytical Chemistry, 48(3), 307-315. (In Chinese)
· Wu, Z.* & Li, Y.* (2020). New frontiers in probing the dynamics of purinergic transmitters in vivo. Neuroscience Research, https://doi.org/10.1016/j.neures.2020.01.008.
· Zeng, J., Sun, F., Wan, J., Feng, J. & Li, Y.* (2019). New optical methods for detecting monoamine neuromodulators. Current Opinion in Biomedical Engineering, https://doi.org/10.1016/j.cobme.2019.09.010.
· Jing, M., Zhang, Y., Wang, H. & Li, Y.* (2019). GPCR‐based sensors for imaging neurochemicals with high sensitivity and specificity. Journal of Neurochemistry, https://doi.org/10.1111/jnc.14855.
· Dong, A.*, Liu, S., & Li, Y.* (2018). Gap Junctions in the Nervous System: Probing Functional Connections Using New Imaging Approaches. Frontiers in Cellular Neuroscience, 12, 320.
· Wang, H., Jing, M., & Li, Y.* (2018). Lighting up the brain: genetically encoded fluorescent sensors for imaging neurotransmitters and neuromodulators. Current Opinion in Neurobiology, 50, 171-178.
· Wang, A.#, Feng, J.#, Li, Y.*, & Zou, P.* (2018). Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities. ACS chemical neuroscience, 9(4), 639-650.
· Qian, C., & Li, Y.* (2015). Spine maturation and pruning during development: Cadherin/Catenin complexes come to help. Science China. Life sciences,58(9), 929.
· Li, Y.*, & Rao, Y.* (2015). Pied Piper of Neuroscience. Cell, 163(2), 267-268.

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