Home /
bzhang(AT)pku.edu.cn
ZHANG, Bo
Title:
Professor
Office Phone: 62759072
Office Address: Jinguang Life Science Building,Peking University, No.5 Yiheyuan Road, Haidian District,Beijing, P.R.China 100871
Lab Phone: 62753077
Lab Address: Jinguang Life Science Building,Peking University, No.5 Yiheyuan Road, Haidian District,Beijing, P.R.China 100871
Lab Homepage: http://
Personal Homepage: http://
Resume
Education
09, 1992 – 07, 1995 PhD. in Cell Biology, Peking University, China
09, 1989 – 07, 1992 Master` Degree in Cell Biology, Peking University, China
09, 1985 – 07, 1989 Bachelor` Degree in Cell Biology, Peking University, China
Professional Experience
08, 2004 – present Professor, College of Life Sciences, Peking University, China
12, 2004 – 08, 2005 Visiting scholar, University of California Los Angeles, U.S.A.
08, 2002 – 07, 2004 Associate professor, College of Life Sciences, Peking University, China
10, 1997 – 07, 2002 Postdoc., Institute of Molecular Biology, University of Zürich, Switzerland
07, 1995 – 09, 1997 Associate professor, College of Life Sciences, Peking University, China
08, 1994 – 02, 1995 Visiting scholar, University of Wisconsin-Madison, USA
Editorial Activities
2021 - present, Frontiers in Cell and Developmental Biology, Review Editor
2020 - present, Journal of Genetics and Genomics, editor
2019 - present, Zebrafish, editor
Grant Review/Study Section Membership
2004–present, National Natural Science Foundation of China, Review Experts
Editorial Activities
2021 - present, Frontiers in Cell and Developmental Biology, Review Editor
2020 - present, Journal of Genetics and Genomics, editor
2019 - present, Zebrafish, editor
Research Interests
By using zebrafish (Danio rerio) as a major animal model, we are interested in: (1) Genetic, cellular and molecular mechanisms of vertebrate embryogenesis and organogenesis, as well as organ regeneration, especially heart development and regeneration. (3) Functional genomics study and technology development: Targeted genome modification/genome editing through TALENs and CRISPR/Cas system; Single-cell transcriptomics. (3) Modeling human diseases in zebrafish and dissecting the underlying genetic, cellular and molecular mechanisms.
Representative Peer-Reviewed Publications
1. Han, B., Zhang, Y., Bi, X., Zhou, Y., Krueger, C. J., Hu, X., Zhu, Z., Tong, X. and Zhang, B., 2020, Bi-FoRe: An efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators. Protein & Cell, 12 (1): 39-56.
2. Li, W., Zhang, Y., Han, B., Li, L., Li, M., Lu, X., Chen, C., Lu, M., Zhang, Y., Jia, X., Zhu, Z., Tong, X. and Zhang, B., 2019, One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish. eLife, 8: e48081.
3. Lu, C.-J., Fan, X.-Y., Guo, Y.-F., Cheng, Z.-C., Dong, J., Chen, J.-Z., Li, L.-Y., Wang, M.-W., Wu, Z.-K., Wang, F., Tong, X.-J., Luo, L.-F., Tang, F.-C., Zhu Z.-Y. and Zhang, B., 2018, Single-cell analyses identify distinct and intermediate states of zebrafish pancreatic islet development. Journal of Molecular Cell Biology, 11 (6): 435–447.
4. Zhang, J., Wang, C., Shen, Y., Chen, N., Wang, L., Liang, L., Guo, T., Yin, X., Ma, Z., Zhang, B. and Yang, L., 2016, A mutation in ADIPOR1 causes nonsyndromic autosomal dominant retinitis pigmentosa. Human Genetics, 135 (12): 1375-1387.
5.Xiao, A. and Zhang, B., 2016, Generation of targeted genomic deletions through CRISPR/Cas system in zebrafish. Methods Mol. Biol., 1451: 65-79.
6. Huang, P., Xiao, A., Tong, X., Lin, S. and Zhang, B., 2016, Targeted Mutagenesis in Zebrafish by TALENs. Methods Mol. Biol., 1338: 191-206.
7. Liu, D., Wang, Z., Xiao, A., Zhang, Y., Li, W., Zu, Y., Yao, S., Lin, S. and Zhang, B., 2014, Efficient gene targeting in zebrafish mediated by a zebrafish-codon-optimized Cas9 and evaluation of off-targeting effect. J. Genet. Genomics, 41: 43-46.
8. Xiao, A., Cheng, Z., Kong, L., Zhu, Z., Lin, S., Gao G. and Zhang, B., 2014, CasOT: a genome-wide Cas9/gRNA off-target searching tool., Bioinformatics, 30 (8): 1180-1182.
9. Huang, P., Xiao, A., Tong, X., Zu, Y., Wang, Z. and Zhang, B., 2014, TALEN construction via Unit Assembly method and targeted genome modifications in zebrafish. Methods, 69 (1): 67-75.
10. Tong, X., Zu, Y., Li, Z., Li, W., Ying, L., Yang, J., Wang, X., He, S., Liu, D., Zhu, Z., Chen, J., Lin, S. and Zhang, B., 2014, Kctd10 regulates heart morphogenesis by repressing the transcriptional activity of Tbx5a in zebrafish. Nat. Commun., 5: 3153.
11. Zu, Y., Tong, X., Wang, Z., Liu, D., Pan, R., Li, Z., Hu, Y., Luo, Z., Huang, P., Wu, Q., Zhu, Z., Zhang, B., Lin, S., 2013, TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nat. Methods, 10 (4): 329-331. (Cover story)
12. Xiao, A., Wu, Y., Yang, Z., Hu, Y., Wang, W., Zhang, Y., Kong, L., Gao, G., Zhu, Z., Lin, S. and Zhang, B., 2013a, EENdb: a database and knowledge base of ZFNs and TALENs for endonuclease engineering. Nucleic Acids Res., 41: D415-D422.
13. Xiao, A., Wang, Z., Hu, Y., Wu, Y., Luo, Z., Yang, Z., Zu, Y., Li, W., Huang, P., Tong, X., Zhu, Z., Lin, S. and Zhang, B., 2013b, Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish. Nucleic Acids Res., 41 (14): e141.
14. Wei, C., Liu, J., Yu, Z., Zhang, B., Gao, G. and Jiao, R., 2013, TALEN or Cas9 - rapid, efficient and specific choices for genome modifications. J. Genet. Genomics, 40 (6): 281-289. (Invited review)
15. Xia, Z., Tong, X., Liang, F., Zhang, Y., Kuok, C., Zhang, Y., Liu, X., Zhu, Z., Lin, S. and Zhang, B., 2013, Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish. Dev. Biol., 381 (1): 83-96.
16. Tong, X., Xia, Z., Zu, Y., Telfer H., Hu, J., Yu, J., Liu, H., Zhang, Q., Sodmergen, Lin, S. and Zhang, B., 2013, Ngs (notochord granular surface) encodes a novel type of intermediate filament family protein essential for notochord maintenance in zebrafish. J. Biol. Chem., 288 (4): 2711-2720.
17. Jiang, L., Zhang, J., Wang, J.-J., Wang, L., Zhang, L., Li, G., Yang, X., Ma, X., Sun, X., Cai, J., Zhang, J., Huang, X., Yu,. M., Wang, X., Liu, F., Wu, C.-I, He, C., Zhang, B., Ci, W. and Liu, J., 2013, Sperm DNA methylome is inherited during zebrafish early embryogenesis. Cell, 153: 773-784.
18. Huang, P., Zhu, Z., Lin, S. and Zhang, B., 2012, Reverse genetic approaches in zebrafish. J. Genet. Genomics, 39 (9): 421-433. (Invited review)
19. Huang, P., Xu, L., Liang, W., Tam, C. I., Zhang, Y., Qi, F., Zhu, Z., Lin, S. and Zhang, B., 2012, Genomic deletion induced by Tol2 transposon excision in zebrafish. Nucleic Acids Res., 41 (2): e36.
20. Huang, P., Xiao, A., Zhou, M., Zhu, Z., Lin, S. and Zhang, B., 2011, Heritable gene targeting in zebrafish using customized TALENs. Nat. Biotechnol., 29 (8): 699-700.
21. Gao, W., Xu, L., Guan, R., Liu, X., Han, Y., Wu, Q., Xiao, Y., Qi, F., Zhu, Z., Lin, S. and Zhang, B., 2011, Wdr18 is required for Kupffer’s vesicle formation and regulation of body asymmetry in zebrafish. PLoS ONE, 6 (8): e23386.
22. Jiang, Z., Song, J., Qi, F., Xiao, A., An, X., Liu, N. A., Zhu, Z., Zhang, B. and Lin S., 2008, Exdpf is a key regulator of exocrine pancreas development controlled by retinoic acid and ptf1a in zebrafish. PLoS Biol., 6 (11): e293.
23. Wei, W., Wen, L., Huang, P., Zhang, Z., Chen, Y., Xiao, A., Huang, H., Zhu, Z., Zhang, B. and Lin, S, 2008, Gfi1.1 regulates hematopoietic lineage differentiation during zebrafish embryogenesis. Cell Res., 18: 677-685.
24. Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S. and Zhang, B., 2008, Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Dev. Biol., 314 (1): 84-92.
25. Wang, D., Jao, L.-E., Zheng, N., Dolan, K., Ivey, J., Zonies, S., Wu, X., Wu, K., Yang, H., Meng, Q., Zhu, Z., Zhang, B., Lin, S. and Burgess, S. M., 2007, Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions. Proc. Natl. Acad. Sci. USA, 104 (30): 12428-12433.
26. Zhang B., Georgiev O., Hagmann M., Günes., Faller P., Vasák M. and Schaffner W, 2003, Activation of metal transcription factor-1 (MTF-1) by toxic heavy metals and H2O2 in vitro is modulated by metallothionein. Mol. Cell. Biol., 23(23): 8471-8485.
27. Zhang B., Egli D., Georgiev O. and Schaffner W., 2001, The Drosophila homolog of mammalian zinc finger factor MTF-1 activates transcription in response to heavy metals. Mol. Cell. Biol., 21(14): 4505-4514.
Teaching
2006–present, Genetics
Laboratory Introduction
By using zebrafish (Danio rerio) as a major animal model, we are interested in: (1) Genetic, cellular and molecular mechanisms of vertebrate embryogenesis and organogenesis, as well as organ regeneration, especially heart development and regeneration. (3) Functional genomics study and technology development: Targeted genome modification/genome editing through TALENs and CRISPR/Cas system; Single-cell transcriptomics. (3) Modeling human diseases in zebrafish and dissecting the underlying genetic, cellular and molecular mechanisms.